- ￥3870 - 29480
- Sino Biological
- 50389-M02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 规格：
50 µg/1 mg
| 规格： | 50 µg | 产品价格： | ￥3870 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥29480 |
蛋白名称：CD8 alpha蛋白, CD8 alpha protein
蛋白构建：A DNA sequence encoding the mouse CD8A (NP_001074579.1) (Met1-Tyr196) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Lys 28
蛋白分子量：The recombinant mouse CD8A consists of 407 amino acids and predicts a molecular mass of 45.6 kDa.
蛋白NP号：NP_001074579.1
蛋白氨基酸序列：Met1-Tyr196
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
【求助】矛盾的实验结果：IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达，结合文献，推理应该是IKb alpha下降，而NFKB升高 （IKb可磷酸化NFKB，使后者降低） 但现在我的结果是两者同时升高，请问可能的原因有哪些呢？ 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况？ erik NFKB是检测核内的吗？检测p65还是p50/ 吴佰霖 检测总蛋白
LABELING ALPHA-ACTININ WITH IATR
.Dialyze overnight against buffer 5 in the cold room. 11.Clarify at 25,000 rpm,4℃,for 20 min in a 42.2Ti rotor. 12.Determine the concentration of alpha-actinin using Lowry assay.Determine dye/protein molar ratio by diluting conjugate 1:40 (10μl in 400μl
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