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- 规格:
100 µg
蛋白名称:TIM-1/KIM-1蛋白, TIM-1/KIM-1 protein
蛋白构建:A DNA sequence encoding the extracellular domain of canine HAVCR1 (XP_854888.1) (Tyr 21-Ser 160) was expressed with a polyhistidine tag at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 96 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:His
蛋白分子量:The recombinant canine HAVCR1 consists of 156 amino acids and has a calculated molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 35-40 kDa due to glycosylation.
蛋白NP号:XP_854888.1
蛋白氨基酸序列:Tyr21-Ser160
蛋白标签:N-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Immunity:厦门大学韩家淮团队揭示 PELO 控制 NOD 样受体家族蛋白寡聚化组装和激活的机制
-associated protein quality control. Nat Rev Mol Cell Bio 20, 368-383 (2019). 5. A. P. Schuller, R. Green, Roadblocks and resolutions in eukaryotic translation. Nat Rev Mol Cell Bio 19, 526-541 (2018). 6. X. R. Wu et al., pelo Is Required for High Efficiency
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
-19]; xChIP:用来研究DNA及低结合力蛋白,采用甲醛或紫外线进行 DNA 和蛋白交联,超声波片段化染色质,然后进行片段富集及后续分析,适用于多数非组蛋白的蛋白,例如 [9, 15, 20]。 X-ChIP 试验的一般过程 以上两种方法在分离 DNA-蛋白质复合物之后,对 DNA 进行 PCR 扩增,验证目标序列的存在。除验证实验外,ChIP DNA也可以进行测序分析,这种方法被称为 ChIP-seq [22] ;也可做芯片分析,这种方法被称为 ChIP on CHIP 或 ChIP
蛋白质纯化的原理和方法(Protein Purification Principles and Methods)
and Hydrophobic-Interactions •“Weak” interactions are important for structure and function (activity) of the protein ÆIn most cases the purification must be gentle! Before the purification… •Cultivation of bacteria •Cell disruption
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