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50 µg
蛋白名称:Factor IX蛋白, Factor IX protein
蛋白构建:A DNA sequence encoding the human F9 (NP_000124.1) (Met1-Thr467) was expressed with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Thr 29
蛋白分子量:The recombinant human F9 consists of 671 amino acids and predicts a molecular mass of 75.5 kDa.
蛋白NP号:NP_000124.1
蛋白氨基酸序列:Met1-Thr467
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Ex Vivo Stromal Cell Electroporation of Factor IX cDNA for Treatment of Hemophilia B
Hemophilia B is an X-linked genetic disorder that typically results from chronic circulating deficiency of blood coagulation factor IX (FIX) (1). While the occurrence of hemophilia B is significantly less frequent than hemophilia A (factor
Identification of Mutations in the Human Factor VII Gene
by activating both arms of the coagulation cascade via its action upon factors IX and X (5 ).
为解决转录因子(TF)染色过程中报告荧光蛋白信号丢失的问题,研究人员首先在CD4-Cre × EYFP小鼠脾细胞中比较了不同固定/破膜方案。进一步实验显示,Fix/Perm处理后的上清液中蛋白含量显著高于Triton X-100处理组,且通过抗GFP抗体捕获法可在Perm处理后的上清中检测到EYFP,但在先经Fix/Perm处理的样本中则无法检出——说明EYFP主要在Fix/Perm步骤中因固定不充分而泄漏。这些结果共同证实:荧光丢失源于固定强度不足,而非荧光团降解。 尽管2%多聚甲醛(PFA
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