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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ 至 -80℃
- 保质期:
12个月
- 英文名:
Enterovirus D68 (EV-D68) (strain Fermon) VP4 Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
100 µg
蛋白名称:EV-D68 VP4蛋白, EV-D68 VP4 protein
蛋白构建:A DNA sequence encoding the VP4 of Enterovirus D68 (EV-D68)(strain Fermon) (AAR98503.1) (Met1-Lys69) was expressed with the Fc region of rabbit IgG at the C-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 95 % as determined by SDS-PAGE.
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg protein as determined by the LAL method.
预测N端:Met
蛋白分子量:The recombinant VP4 of Enterovirus D68 (EV-D68)(strain Fermon) consists of 293 amino acids and predicts a molecular mass of 32.7 kDa.
蛋白NP号:AAR98503.1
蛋白氨基酸序列:Met1-Lys69
蛋白标签:C-rabbit IgG-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验自杀性质粒载体:一般用于基因突变。将突变的目的基因克隆到自杀性质粒载体上,通过接合等使其进入宿主,由于在宿主菌中不存在复制基因启始所需的复制蛋白(如Pi蛋白等),其无法复制,在外界选择性压力的作用下,自杀性质粒载体所携带的突变基因就与宿主染色体上的野生型发生基因发生二次同源重组,产生了带有突变的突变株;而质粒载体自身由于自杀性特性连同染色体上原有的野生型基因一起随着细菌的传代从菌体内消失。至于一次重组的突变株,可借助质粒上的某些选择基因(如sacB,sucrose敏感)筛除。整合载体:将目的
Transcriptome Profiling of HostMicrobe Interactions by Differential Display RT-PCR
cells to infection with a neurovirulent strain of enterovirus 71 (EV71) at different time-points during the infection process compared with uninfected cells. Using differential display RT-PCR, we identified mRNAs that were up- or down-regulated
【求助】我在genebank提交核苷酸序列遇到困难,请求帮助
enterovirus 71 strain NBChina01 from China,complete cds. ACCESSION VERSION KEYWORDS . SOURCE Human enterovirus 71 ORGANISM Human enterovirus 71 Viruses; ssRNA positive-strand viruses, no DNA stage; Picornavirales; Picornaviridae
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