• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        【求助】我在genebank提交核苷酸序列遇到困难,请求帮助

        丁香园论坛

        2926
        我在genebank提交核苷酸序列遇到困难,请求园内老师帮助,自己花费很大精力,可是还是存在问题。
        [1] Do you know the correct annotation for your sequence(s)? If so,
        please resubmit your sequence(s) with relevant features such as:

          - coding regions (CDS features) including nucleotide spans
            and reading frame. Using this information, our software
            will add the amino acid translations for you.
          - structural RNAs such as rRNAs, tRNAs, misc_RNAs, with
            nucleotide spans
          - features which may describe your sequence, such as
          repeat_regions, UTRs, promoters with nucleotide spans

        【2】下面是我提交的相关信息:
        preliminary GenBank flatfiles:

        LOCUS NBChina01 7414 bp RNA linear VRL 03-DEC-2010
        DEFINITION Human enterovirus 71 strain NBChina01 from China,complete cds.
        ACCESSION
        VERSION
        KEYWORDS .
        SOURCE Human enterovirus 71
        ORGANISM Human enterovirus 71
        Viruses; ssRNA positive-strand viruses, no DNA stage;
        Picornavirales; Picornaviridae; Enterovirus; Human enterovirus A.
        REFERENCE 1 (bases 1 to 7414)
        AUTHORS Hu,A.-r., Gu,W.-z. and Jiang,S.-w.
        TITLE Autopsy report and complete genome sequence analysis of one case
        with critical hang, foot and mouth disease(HFMD) infected by
        enterovirus 71(EV71)
        JOURNAL Unpublished
        REFERENCE 2 (bases 1 to 7414)
        AUTHORS Hu,A.-r., Gu,W.-z. and Jiang,S.-w.
        TITLE Direct Submission
        JOURNAL Submitted (03-DEC-2010) Science and Education Division, Ningbo
        Infectious Diseases Hospital, No175,Yongfeng Road(North), Ningbo
        City, Zhejiang Province 315016, China
        COMMENT Bankit Comment: bankit1414927.
        Bankit Comment: BankIt ID:1414927.
        Bankit Comment: LocalID:NBChina01.
        FEATURES Location/Qualifiers
        source 1..7414
        /organism="Human enterovirus 71"
        /proviral
        /mol_type="genomic RNA"
        /db_xref="taxon:39054"
        /PCR_primers="fwd_name: F1, fwd_seq: ttaaaacagctgtgggttg,
        fwd_name: F2, fwd_seq: atagtcggctatggtgagtg, fwd_name: F3,
        fwd_seq: tgttcactgggtcctttatggc, fwd_name: F4, fwd_seq:
        taccattcatgtcacctgcga, fwd_name: F5, fwd_seq:
        gttttcagagcacagtttagcgg, fwd_name: F6, fwd_seq:
        gtcttccagtttctttattgggctt, fwd_name: F7, fwd_seq:
        ttcattctactcacgtccct, fwd_name: F8, fwd_seq:
        gatcttgagtgtttcgtgcgtc, fwd_name: 3'-terminus, fwd_seq:
        gacctattgtgtgcttggtgggatg, rev_name: R1, rev_seq:
        acttccagtaccatcccttg, rev_name: R2, rev_seq:
        agtgagtgttgctgatccatggt, rev_name: R3, rev_seq:
        accagcataatttgggttggct, rev_name: R4, rev_seq:
        ctagatacgacacattcccaaa, rev_name: R5, rev_seq:
        gtcggcatagtgtctactggtg, rev_name: R6, rev_seq:
        tgtcaaatggtatccaccgtag, rev_name: R7, rev_seq:
        atcgcttagcagtcctccca, rev_name: R8, rev_seq:
        cggtactgagaatcttgaggct, rev_name: 5'-terminus, rev_seq:
        ggtatgtgctccgcagttagg"
        /note="/organism='Human enterovirus
        71'##/mol_type='genomic
        RNA'##/strain='NBChina01'##/isolation_source='clinical
        specimen'##/country='China:
        Ningbo'##/collection_date='August-2010'"
        BASE COUNT 2018 a 1791 c 1767 g 1838 t
        ORIGIN
        1 ttaaaacagc ctgtgggttg cacccactca cagggcccac tgggcgctag cactctggta
        61 tcttggtacc tttgtgcgcc tgttttatac ccccccccag tgaaacttag aagcagcaaa
        121 cctcgatcaa tagcaggcat aacgctccag ttatgtcttg atcaagcact tctgtttccc
        181 cggactgagt atcaatagac tgctcgcgcg gttgaaggag aaaacgttcg ttatccggct
        241 aactacttcg gaaaacctag taacaccatg gaagttgcgg agagctccgt tcagcactcc
        301 cccagtgtag atcaggtcga tgagtcaccg cattccccac gggcgaccgt ggcggtggct
        361 gcgttggcgg cctgcccatg gggtaaccca tggggcgctc taatacagac atggtgtgaa
        421 gagtctactg agctagttag tagtcctccg gcccctgaat gcggctaatc ccaactgcgg
        481 agcacacgcc cacaagccag cgggtagtgt gtcgtaacgg gtaactctgc agcggaaccg
        541 actactttgg gtgtccgtgt ttccttttat ctttacattg gctgcttatg gtgacaatta
        601 aagaattgtt accatatagc tattggattg gccatccggt gtgcaacaga gcaattattt
        661 acctatttat tggttttgta ccatcaactt tgaattctgt gaccaccctt aattatatcc
        721 tgacccttaa cacagctaaa catgggttcg caagcgtcta cacagcgctc cggttcccac
        781 ggaaattcaa actcagccac tgagggttct accataaact acaccactat caattactac
        841 aaagactcct atgctgccac agcaggcaaa cagagtctca agcaggatcc agacaagttt
        901 gcaaatcctg ttaaatacat cttcactgaa atggcagcgc cactgaaatc cccatccgct
        961 gaggcatgtg ggtacagtga tcgagtggcg caattaacta ttggcaactc cactatcacc
        1021 acgcaagaag cggctaacat catagtcggt tatggcgaat ggccttccta ctgctcagat
        1081 tctgacgcta cagcagtgga taaaccaacg cgcccggatg tttcagtgaa caggttttac
        1141 acattggaca ctaaattgtg ggagaaatcg tccaagggat ggtactggaa gttcccggat
        1201 gtgttaactg aaactggggt ttttgggcaa aatgcacaat tccactacct ctaccgatca
        1261 gggttctgca tccacgtaca gtgcaatgcc agtaaattcc atcaaggagc actcctagtc
        1321 gctgtcctac cagagtatgt cattgggaca gtggcaggcg gtacagggac ggaagatacc
        1381 cacccccctt ataaacagac tcaacccggc gccgatggtt tcgagttgca acacccgtac
        1441 gtgcttgatg ctggcatccc aatatcacag ttaacagtgt gcccacacca gtggatcaat
        1501 ctaaggacca acaattgtgc tacaataata gtgccataca ttaacgcact gccttttgat
        1561 tctgccttga atcattgcaa ctttggcctg ttagttgtgc ctattagccc actagactac
        1621 gaccaaggag cgacgccagt aatccctata actattacat tggccccaat gtgttctgaa
        1681 ttcgcaggtc ttaggcaggc agtcacgcaa gggttcccca ccgagctaaa acctggcaca
        1741 aaccaatttt taaccaccga tgatggcgtt tcagcaccta ttctaccaaa cttccacccc
        1801 accccgtgta tccacatacc tggtgaagtt aggaacttgc tagagttatg ccaggtggag
        1861 accattctgg aggttaacaa tgtgcccacg aatgccacta gcttaatgga gagactgcgc
        1921 ttcccggtct cagcacaagc agggaagggt gagctgtgtg cggtgtttag agccgatcct
        1981 gggcgaagtg gaccatggca atccacctta ctgggccagt tgtgcgggta ctacacccaa
        2041 tggtcaggat cattggaagt taccttcatg tttactggat ccttcatggc taccggcaag
        2101 atgctcatag cctatacacc gccaggaggt cctctgccca aggaccgggc gaccgccatg
        2161 ttgggcacgc acgtcatctg ggattttggg ctgcaatcgt ctgttaccct tgtaatacca
        2221 tggatcagca acactcatta tagagcacat gcccgagatg gggtgtttga ctactacacc
        2281 acagggttag tcagtatatg gtatcagaca aattatgtgg ttccaatcgg tgcgcccaac
        2341 acagcctata taatagcatt agcggcagct caaaagaact tcactatgaa attgtgcaag
        2401 gatgctagtg acatcctaca gacgggcacc atccagggag atagggtggc agatgtaata
        2461 gaaagctcca taggagatag cgtgagcaga gccctcactc acgctctacc agcacccaca
        2521 ggccagaaca cacaggtgag cagtcatcga ctggatacag gtaaggttcc agcactccaa
        2581 gctgctgaaa ttggagcatc atcaaatgct agtgacgaga gcatgattga gacacgctgt
        2641 gttcttaact cgcacagtac agctgagacc actcttgata gtttcttcag cagggcggga
        2701 ttagttggag agatagatct ccctcttgag ggcacaacta acccaaatgg ttatgccaac
        2761 tgggacatag atataacagg ttacgcgcaa atgcgtagaa aggtagagct attcacctac
        2821 atgcgctttg atgcagagtt cacttttgtt gcgtgcacac ccaccgggga agttgttcca
        2881 caaatgctcc aatatatgtt tgtgccacct ggagccccta agccagattc aagggaatcc
        2941 cttgcatggc aaactgccac taacccctca gtttttgtca agctgtcaga ccctccagcg
        3001 caggtttcag tgccattcat gtcacctgcg agtgcttatc aatggtttta tgacggatat
        3061 cccacattcg gggaacacaa acaggagaaa gatcttgaat acggggcatg tcctaataac
        3121 atgatgggca cgttctcagt gcggactgta gggacctcca agtccaagta ccctttagtg
        3181 gttaggatct acatgagaat gaagcacgtc agggcgtgga tacctcgccc gatgcgtaac
        3241 cagaactacc tattcaaagc caacccaaat tatgctggca actccattaa gccaactggt
        3301 gctagtcgca cagcgatcac cactcttggg aaatttggac aacagtctgg ggctatttat
        3361 gtgggcaact ttagagtggt caaccgacat cttgccactc acaatgattg ggcaaatctt
        3421 gtttgggaag acagctctcg cgacttgctt gtgtcgtcca ccaccgccca aggttgtgac
        3481 acgattgctc gctgcgattg ccagacaggg gtgtactact gtaattcgat gagaaaacac
        3541 tacccagtca gtttttcaaa acccagcctg atctatgtag aggctagcga gtattaccca
        3601 gccaggtacc aatcacatct catgctcgca cagggtcact cagaacctgg tgattgcggt
        3661 ggtatcctta gatgccaaca tggcgtcgtc ggcatagtgt ctactggtgg taatgggctc
        3721 gttggctttg cagacgtcag agacctcttg tggttagatg aagaagctat ggaacagggc
        3781 gtgtccgact acatcaaggg tctcggagat gcttttggaa caggcttcac tgacgcagtc
        3841 tcaagggagg ttgaagctct caaaaactat cttatagggt ctgaaggagc agttgagaaa
        3901 atcttgaaaa atcttattaa actaatctct gcactggtga ttgtgatcag aagtgattac
        3961 gacatggtta ccctcactgc aaccttagcg ctgataggtt gtcatggcag tccttgggct
        4021 tggatcaaag ccaaaacagc ctccatctta ggtatcccta tcgctcaaaa gcagagcgct
        4081 tcctggctca agaagttcaa tgacatggcc aacgccgcta aggggttaga gtgggtttcc
        4141 aacaagatca gcaaatttat tgattggctt aaggagaaaa tagtaccagc ggccagggag
        4201 aaggttgaat tcctaaataa cttgaaacag ctgccactgc tagagaatca gatctcaaac
        4261 ttggaacagt ctgctgcctc acaagaggat cttgaagtca tgtttgggaa tgtgtcgtac
        4321 ctagctcact tctgtcgcaa gttccaaccg ctatacgcca cggaagctaa aagagtctat
        4381 gccctggaga agagaatgaa taactatatg cagttcaaga gcaaacaccg aattgaacct
        4441 gtatgtctca ttattagggg ctcaccaggc actgggaagt ctctagccac tggtattatt
        4501 gctcgagcaa ttgctgataa gtaccactcc agcgtgtact cgcttccacc agacccggat
        4561 cattttgacg gttacaagca acaggtggtt acagtgatgg atgatttgtg tcaaaacccc
        4621 gatggtaagg atatgtcttt attctgtcaa atggtatcca ccgtagactt cattccacca
        4681 atggcttctc tcgaggagaa gggagtttcc ttcacctcta agtttgtcat cgcatccact
        4741 aatgccagta atatcatagt accaacagtg tctgattctg acgctattcg ccgcaggttc
        4801 tacatggact gtgacattga agtgacagac tcgtacaaaa cagatctagg tagactggat
        4861 gcagggcgag ccgctaaact gtgctctgaa aacaacactg caaatttcaa acgttgcagc
        4921 ccattagtgt gtgggaaagc tatccaactt agagatagaa agtctaaagt cagatacagt
        4981 gtggatacgg tggtttcaga actcattagg gaatacagca ataggtccgc tattggcaac
        5041 acaatcgagg ctcttttcca aggtccaccc aagttcaggc caattaggat tagccttgaa
        5101 gaaaaaccag ccccagacgc tattagcgat ctccttgcta gcgtagatag tgaagaagtg
        5161 cgccagtact gcagggatca aggctggatt attcctgaag ctcccaccaa tgtagagcgg
        5221 caccttaata gagcggtgct tgtcatgcaa tccatcgcca cagtagtggc ggttgtctcg
        5281 ttggtgtatg tcatctacaa gctctttgca gggttccagg gtgcgtattc tggtgctcct
        5341 aagcaagtgc ttaagaaacc tgctcttcgc acagcgacag tacagggccc gagccttgac
        5401 tttgctctct ccctactgag aaggaacatc aggcaagtcc aaacagacca ggggcatttc
        5461 accatgttgg gtgttaggga tcgcttagca gtcctcccac gccactcaca acctggtaaa
        5521 actatttggg ttgagcacaa actcgtgaac gtccttgatg cagttgaact ggtggatgag
        5581 caaggagtca acctggaatt aaccctcatt actcttgaca ccaacgaaaa gtttagggat
        5641 atcaccaaat tcatcccaga aaatattagc actgctagtg atgccaccct agtgatcaac
        5701 acggagcaca tgccatcaat gtttgtcccg gtgggtgacg ttgtgcagta tggctttttg
        5761 aatctcagtg gtaagcctac ccatcgcacc atgatgtaca actttcctac taaagcagga
        5821 cagtgtggag gagtggtgac atctgtcggg aaggttgtcg gcattcacat tggtggcaat
        5881 ggcagacaag gtttttgcgc aggcctcaaa aggagttact ttgccagtga acaaggagag
        5941 atccagtggg ttaagcccaa taaagaaact ggaagactca acatcaatgg acctacccgc
        6001 accaagttag aacctagtgt attccatgac atcttcgagg gaaataaaga accagctgtc
        6061 ttgcacagta aagacccccg acttgaggta gactttgaac aggccctgtt ctctaagtat
        6121 gtgggaaaca cactacatga gcctgacgag tacatcaaag aggcagctct tcattatgca
        6181 aaccaattaa agcaactaga aattaatacc tctcaaatga gcatggagga ggcctgctac
        6241 ggtactgaga atcttgaggc tattgatctt cacactagtg caggttaccc ctacagtgcc
        6301 ctggggataa agaaaagaga catcttagac cctaccacca gggacgtgag tagaatgaag
        6361 ttctacatgg acaagtatgg tcttgatctt ccttactcca cttatgtcaa ggacgagctg
        6421 cgctcgattg ataaaatcaa gaaagggaag tcccgtctga tcgaggccag tagtctaaat
        6481 gattcagtgt acctcagaat ggctttcgga catttgtatg aggctttcca cgcaaatcct
        6541 gggacgataa ctggatcagc cgtggggtgt aaccctgaca cattctggag caagctgcca
        6601 attttgctcc ctggttcact ctttgccttt gactactcag gttatgatgc tagccttagc
        6661 cctgtctggt tcagagcatt agaattggtt cttagggaga tagggtatag tgaagaggca
        6721 gtctcactca ttgagggaat caaccacaca catcatgtgt atcgtaataa gacctattgt
        6781 gtgcttggtg ggatgccctc aggctgttcg ggaacgtcca ttttcaactc aatgatcaat
        6841 aacattatta tcagagcact gctcgtaaaa acatttaagg gcattgattt ggatgaactc
        6901 aacatggttg cttatggaga cgatgtgctc gctagctatc cctacccaat tgattgcttg
        6961 gaactagcaa agactggtaa ggagtatggt ctgaccatga ctcctgctga taaatctcct
        7021 tgctttaatg aggtcaattg gggtaatgcg accttcctca aaaggggctt cttgcccgat
        7081 gaacagtttc catttttgat tcaccctact atgccaatga gtgagatcca tgagtccatt
        7141 cgatggacca gggacgcacg aaacactcaa gatcatgtgc ggtccttgtg cctcctagca
        7201 tggcataatg gtaagcaaga atatgagaag tttgtgagca caattaggtc tgtcccagta
        7261 ggaagagcgt tggctatccc aaattatgaa aatcttagac gcaattggct cgaattattt
        7321 tagaggttat acacacctca accccaccag aaatctggtc gtgaatgtga ctggtggggg
        7381 taaatttgtt ataaccagaa tagcaaaaaa aaaa
        //

        【3】如果老师们可以帮助,请直接回复或者PM与我您的联系方式,不甚感谢!
        如果老师们可以帮助,请直接回复或者PM与我您的联系方式,不甚感谢!
        hehe,是要你提供序列注释。你如何得到该序列的?ORF起止位置?有没有重复区?有没有非翻译区?有没有启动子?这样大家用你提供的序列才能方便。
        其实你如果按BANKIT活SEQIN的步骤一步步来,应该不会漏掉的。你现在可通过RESUBMISSION修改。
        如果实在有困难可直接回信请求帮助,要求他们帮你。

        本文由丁香园论坛提供,想了解更多有用的、有意思的前沿资讯以及酷炫的实验方法的你,都可以成为师兄的好伙伴

        师兄微信号:shixiongcoming

        【求助】我在genebank提交核苷酸序列遇到困难,请求帮助

        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序