- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-26083r
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Ephrin-A5,Efna5
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Ephrin- A5, Efna5 ELISA KIT
Product Name:Rat Ephrin- A5, Efna5 ELISA KIT
Packing:96T
Catalog No.:ELI-26083r
Gene Name:Efna5
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Rat Ephrin- A5
Alternative Name:Efna5,Rat Ephrin- A5
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat Ephrin- A5, Efna5 ELISA KIT allows for the in vitro quantitative determination ofRat Ephrin- A5 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Rat Ephrin- A5, Efna5 ELISA KIT has been pre-coated with an Efna5 antibody specific to Rat Ephrin- A5 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rat Ephrin- A5 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Ephrin- A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rat Ephrin- A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验. These proteins were enriched in the tumour endothelial plasma membranes relative to tumour homogenates, consistent with proper subfractionation. Eight new tumour-induced vascular proteins were also identified: annexin A1; annexin A8; ephrin A5; ephrin A7
过程中，一抗应在封闭缓冲液 (TBS/0.3%Triton. X–100/5% NGS) 中稀释。抗体：Phospho–Akt (Ser473) (D9E) XP. Rabbit mAb #4060、Phospho–EGFReceptor (Tyr1173) (53A5) Rabbit mAb #4407样本：石蜡包埋人类乳腺肿瘤（上图）和 HCC827 异种移植物（下图）抗体稀释：SignalStain. 抗体稀释液（左）或 TBST/5%NGS（右）检测—检测系统传统的 IHC 检测
Intracellular Immunofluorescent Staining for Flow Cytometry
for detection of IL-6-producing cells by human LPS-activated monocytes is 6 hours, whereas IL-10 detection needs at least 24 hours stimulation. In contrast to detection of secreted cytokines by ELISA, for detection of intracellular cytokines
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