- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-21685r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Phospholipase D3,Pld3
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Phospholipase D3, PLD3 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PLD3,Phospholipase D3,AD19; HU-K4; HUK4; choline phosphatase 3; hindIII K4L homolog; phosphatidylcholine-hydrolyzing phospholipase D3; phospholipase D family, member 3; phospholipase D family member 3
Search name
Rat PLD3 ELISA KIT ,Rat Phospholipase D3 ELISA KIT ,Rat AD19 ELISA KIT ,Rat HU-K4 ELISA KIT ,Rat HUK4 ELISA KIT ,Rat choline phosphatase 3 ELISA KIT ,Rat hindIII K4L homolog ELISA KIT ,Rat phosphatidylcholine-hydrolyzing phospholipase D3 ELISA KIT ,Rat phospholipase D family ELISA KIT ,Rat member 3 ELISA KIT ,Rat phospholipase D family member 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Phospholipase D3, PLD3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Phospholipase D3,PLD3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rat Phospholipase D3,PLD3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rat Phospholipase D3,PLD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat Phospholipase D3,PLD3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
) 1 (Hmox1), mRNA. ACCESSION NM_012580 VERSION NM_012580.2 GI:61098187 KEYWORDS . SOURCE Rattus norvegicus (Norway rat) ORGANISM Rattus norvegicus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires
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