Zona pellucida-binding protein
1, Zpbp ELISA KIT/ Mouse Zona pellucida-binding protein 1, Zpbp ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-51186m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Zona pellucida-binding protein 1, Zpbp
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT
Product Name:Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT
Packing:96T
Catalog No.:ELI-51186m
Gene Name:Mouse Zpbp
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Zpbp
Alternative Name:Mouse Zona pellucida-binding protein 1, Zpbp
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT allows for the in vitro quantitative determination of Mouse Zpbp concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Zona pellucida- binding protein 1, Zpbp ELISA KIT has been pre-coated with an Mouse Zona pellucida-binding protein 1, Zpbp antibody specific to Mouse Zpbp .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Zpbp and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Zpbp , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Zpbp in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验pellucida appear to be relatively species-specific (1 ,2 ) and have been studied in most detail in the mouse. The mouse zona pellucida is composed of three glycoproteins termed ZP1, ZP2, and ZP3 in order of decreasing molecular weight (3 ).
Free-Hand Bisection of Mouse Oocytes and Embryos
functions and by Western blot and genetic analysis (e.g., RT-PCR). Here we describe a detailed protocol for the free-hand bisection of mouse zona pellucida -free oocytes and embryos on an agar layer using a glass needle.
Generation of Chimeras by Morula Aggregation
embryo (3.5 dpc) ES cells can be brought together with diploid morula-stage embryos (2.5 dpc). The zona pellucida of the embryo needs to be removed and one or two embryos (sandwich aggregation) are put together with ES cells into an indentation
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