Ras GTPase-activating protein
2, Rasa2 ELISA KIT/ Mouse Ras GTPase-activating protein 2, Rasa2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-44295m
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ras GTPase-activating protein 2, Rasa2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ras GTPase- activating protein 2, Rasa2 ELISA KIT
Product Name:Mouse Ras GTPase- activating protein 2, Rasa2 ELISA KIT
Packing:96T
Catalog No.:ELI-44295m
Gene Name:Mouse Rasa2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Rasa2
Alternative Name:Mouse Ras GTPase-activating protein 2, Rasa2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ras GTPase- activating protein 2, Rasa2 ELISA KIT allows for the in vitro quantitative determination of Mouse Rasa2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ras GTPase- activating protein 2, Rasa2 ELISA KIT has been pre-coated with an Mouse Ras GTPase-activating protein 2, Rasa2 antibody specific to Mouse Rasa2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rasa2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rasa2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rasa2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验上释放出来,取而代之的是GTP,从而将Ras激活,GEF的活性受生长因子及其受体的影响。另一个控制Ras蛋白活性的是GTP酶激活蛋白(GTPase activating protein, GAP),存在于正常细胞中,主要作用是激活Ras蛋白的GTP酶，将结合在Ras蛋白上的 GTP水解成GDP，成为失活型的 Ras蛋白—GDP。所以在正常情况下，Ras蛋白基本上都与 GDP结合在一起，定位在细胞质膜内表面上。
, which are active in the GTP-bound form and inactive in the GDP-bound form. Given the importance of studies on the Ras protein activation state to understand the detailed mechanism of Ras-mediated signal transduction, we provide here a simple
Rac and Rap GTPase Activation Assays
The detection of Ras superfamily GTPase activity in neutrophils is important when studying signaling events elicited by various ligands and cellular processes. Substantial progress in monitoring GTPase activation has been made in recent years
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