Mouse IRG1 (Immune- responsive

Mouse IRG1 (Immune- responsive gene 1 protein) ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

Alternative name

Mouse IRG1 ELISA KIT ,Mouse CAD ELISA KIT ,Mouse aconitate decarboxylase ELISA KIT ,Mouse cis-aconitate decarboxylase ELISA KIT ,Mouse cis-aconitic acid decarboxylase ELISA KIT ,Mouse immune-responsive gene 1 protein homolog ELISA KIT ,Mouse immunoresponsive 1 homolog ELISA KIT

Intended use

Mouse IRG1 ELISA kit allows for the in vitro quantitative determination of Mouse Immune- responsive gene 1 protein concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

Mouse IRG1 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IRG1, During the reaction, IRG1 in the sample or standard competes with a fixed amount of biotin-labeled IRG1 for sites on a pre-coated Monoclonal antibody specific to IRG1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IRG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.