- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-45924m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Prohibitin, Phb
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Prohibitin, Phb ELISA KIT
Product Name:Mouse Prohibitin, Phb ELISA KIT
Packing:96T
Catalog No.:ELI-45924m
Gene Name:Mouse Phb
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Phb
Alternative Name:Mouse Prohibitin, Phb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Prohibitin, Phb ELISA KIT allows for the in vitro quantitative determination of Mouse Phb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Prohibitin, Phb ELISA KIT has been pre-coated with an Mouse Prohibitin, Phb antibody specific to Mouse Phb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Phb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Phb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Phb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验人 阻抑素（PHB） 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 阻抑素（PHB）的 含量。 实验原理 ： 本试剂盒应用 双抗体 夹心法测定 标本 中 人阻抑素（PHB ） 水平。用纯化的 人阻抑素（PHB ）抗体 包被微孔板，制成固相 抗 体，往包被 单抗 的微孔中依次加入 阻抑素（PHB ） ，再与HRP 标记的阻抑素（ PHB ）抗体结合，形成抗体
外膜上，而后与LC3II结合介导线粒体自噬。来自武汉大学生命科学学院宋质银教授研究组发表了题为“PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis （PHB2通过PRAL-PGAM5-PINK1轴线促进PINK1-Parkin介导的线粒体自噬）”的最新研究成果，揭示了PHB2调节线粒体自噬的新的分子机制，而且发现了以PHB2为靶点通过阻止线粒体自噬来抑制肿瘤细胞的生存可作
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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