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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
GSK-3-binding protein FRAT2, Frat2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse GSK- 3- binding protein FRAT2, Frat2 ELISA KIT
Product Name:Mouse GSK- 3- binding protein FRAT2, Frat2 ELISA KIT
Packing:96T
Catalog No.:ELI-38335m
Gene Name:Mouse Frat2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Frat2
Alternative Name:Mouse GSK-3-binding protein FRAT2, Frat2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse GSK- 3- binding protein FRAT2, Frat2 ELISA KIT allows for the in vitro quantitative determination of Mouse Frat2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse GSK- 3- binding protein FRAT2, Frat2 ELISA KIT has been pre-coated with an Mouse GSK-3-binding protein FRAT2, Frat2 antibody specific to Mouse Frat2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Frat2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Frat2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Frat2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
. A physicochemical study f protein G, a molecule with unique immunoglobulin G-binding properties. J. Biol. Chem. 261:10240-10247. Harlow, E. and Lane, D. 1988. Antibodies. A laboratory manual. Cold Spring Harbor Laboratory. Kronvall, G., Seal, U. S
Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
DNase I Footprint Analysis of Protein‐DNA Binding
I (DNase I) protection mapping, or footprinting, is a valuable technique for locating the specific binding sites of proteins on DNA. The basis of this assay is that bound protein protects the phosphodiester backbone of DNA from DNase I?catalyzed hydrolysis
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