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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
RNA-binding protein Nova-1, Nova1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse RNA- binding protein Nova- 1, Nova1 ELISA KIT
Product Name:Mouse RNA- binding protein Nova- 1, Nova1 ELISA KIT
Packing:96T
Catalog No.:ELI-22111m
Gene Name:Mouse Nova1
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Nova1
Alternative Name:Mouse RNA-binding protein Nova-1, Nova1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse RNA- binding protein Nova- 1, Nova1 ELISA KIT allows for the in vitro quantitative determination of Mouse Nova1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse RNA- binding protein Nova- 1, Nova1 ELISA KIT has been pre-coated with an Mouse RNA-binding protein Nova-1, Nova1 antibody specific to Mouse Nova1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nova1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nova1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nova1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
实验原理 If using the SQ DNA/RNA/Protein for the first time, please read this booklet to become familiar with the procedure and its various modifications. Samples are first lysed in a specially formulated buffer. The protein
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
Table I Species Protein A Protein G Human ++++ ++++ Mouse ++ ++ Rat
CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins
We present a newly developed method for fixing RNA-protein complexes in situ in living cells and the subsequent purification of the RNA targets. Using this approach, complex tissue such as mouse brain can be ultraviolet (UV) irradiated
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