Mouse X- linked retinitis pigmentosa GTPase regulator, Rpgr ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse Rpgr ELISA KIT ,Mouse COD1 ELISA KIT ,Mouse CORDX1 ELISA KIT ,Mouse CRD ELISA KIT ,Mouse PCDX ELISA KIT ,Mouse RP15 ELISA KIT ,Mouse RP3 ELISA KIT ,Mouse XLRP3 ELISA KIT ,Mouse orf15 ELISA KIT ,Mouse retinitis pigmentosa 15 ELISA KIT ,Mouse retinitis pigmentosa 3 GTPase regulator ELISA KIT ,Mouse retinitis pigmentosa GTPase regulator ELISA KIT
Intended use
Mouse Rpgr ELISA kit allows for the in vitro quantitative determination of Mouse X- linked retinitis pigmentosa GTPase regulator concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse Rpgr ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rpgr, During the reaction, Rpgr in the sample or standard competes with a fixed amount of biotin-labeled Rpgr for sites on a pre-coated Monoclonal antibody specific to Rpgr. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rpgr in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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