RNA-binding protein FUS, Fus E

96 Tests Operating instruction

Alternative name

Mouse FUS ELISA KIT ,Mouse FUS RNA binding protein ELISA KIT ,Mouse oncogene FUS ELISA KIT ,Mouse fus-like protein ELISA KIT ,Mouse ALS6 ELISA KIT ,Mouse ETM4 ELISA KIT ,Mouse FUS1 ELISA KIT ,Mouse HNRNPP2 ELISA KIT ,Mouse POMP75 ELISA KIT ,Mouse TLS ELISA KIT ,Mouse 75 kDa DNA-pairing protein ELISA KIT ,Mouse fused in sarcoma ELISA KIT ,Mouse fusion gene in myxoid liposarcoma ELISA KIT ,Mouse heterogeneous nuclear ribonucleoprotein P2 ELISA KIT ,Mouse oncogene TLS ELISA KIT ,Mouse translocated in liposarcoma protei ELISA KIT ,Mouse RNA-binding protein FUS ELISA KIT

Intended use

Mouse FUS ELISA KIT allows for the in vitro quantitative determination of mouse RNA-binding protein FUS concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse FUS ELISA KIT has been pre-coated with an antibody specific to RNA-binding protein FUS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forRNA-binding protein FUS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RNA-binding protein FUS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of RNA-binding protein FUS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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