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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Keratin, type II cytoskeletal 1b, Krt77含量
- 适应物种:
小鼠
- 标记物:
Keratin, type II cytoskeletal 1b, Krt77
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Keratin, type II cytoskeletal 1b, Krt77 ELISA KIT
Product Name:Mouse Keratin, type II cytoskeletal 1b, Krt77 ELISA KIT
Packing:96T
Catalog No.:ELI-08067m
Gene Name:Mouse Krt77
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Krt77
Alternative Name:Mouse Keratin, type II cytoskeletal 1b, Krt77
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Keratin, type II cytoskeletal 1b, Krt77 ELISA KIT allows for the in vitro quantitative determination of Mouse Krt77 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Keratin, type II cytoskeletal 1b, Krt77 ELISA KIT has been pre-coated with an Mouse Keratin, type II cytoskeletal 1b, Krt77 antibody specific to Mouse Krt77 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Krt77 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Krt77 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Krt77 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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