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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
PR domain zinc finger protein 1, Prdm1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse PR domain zinc finger protein 1, Prdm1 ELISA KIT
Product Name:Mouse PR domain zinc finger protein 1, Prdm1 ELISA KIT
Packing:96T
Catalog No.:ELI-12773m
Gene Name:Mouse Prdm1
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Prdm1
Alternative Name:Mouse PR domain zinc finger protein 1, Prdm1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse PR domain zinc finger protein 1, Prdm1 ELISA KIT allows for the in vitro quantitative determination of Mouse Prdm1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse PR domain zinc finger protein 1, Prdm1 ELISA KIT has been pre-coated with an Mouse PR domain zinc finger protein 1, Prdm1 antibody specific to Mouse Prdm1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Prdm1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Prdm1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Prdm1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Gene Editing in One‐Cell Embryos by Zinc‐Finger and TAL Nucleases
. Cui, X., Ji, D., Fisher, D.A., Wu, Y., Briner, D.M., and Weinstein, E.J. 2011. Targeted integration in rat and mouse embryos with zinc‐finger nucleases. Nat. Biotechnol. 29:64‐67
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
harboring the plasmid that encodes the QSSR1 zinc finger fused to fingers 1 and 2 of the Zif268 protein and the Gal4 activation domain formed blue colonies only when mated with cells that contained the GTA and GCA reporter plasmids. This means
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