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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Interleukin-20, Il20含量
- 适应物种:
小鼠
- 标记物:
Interleukin-20, Il20
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Interleukin- 20, Il20 ELISA KIT
Product Name:Mouse Interleukin- 20, Il20 ELISA KIT
Packing:96T
Catalog No.:ELI-06600m
Gene Name:Mouse Il20
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Il20
Alternative Name:Mouse Interleukin-20, Il20
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Interleukin- 20, Il20 ELISA KIT allows for the in vitro quantitative determination of Mouse Il20 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Interleukin- 20, Il20 ELISA KIT has been pre-coated with an Mouse Interleukin-20, Il20 antibody specific to Mouse Il20 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Il20 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Il20 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Il20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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0.05% Tween-20 for 2 minutes, each time. 4) Proceed immediately to Signal Development, below. Signal Development 1) Dilute the 20X DAB Chromogen supplied with the kit in a sterile microcentrifuge tube just prior to use
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