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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Centromere protein C 1, Cenpc1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Centromere protein C 1, Cenpc1 ELISA KIT
Product Name:Mouse Centromere protein C 1, Cenpc1 ELISA KIT
Packing:96T
Catalog No.:ELI-50572m
Gene Name:Mouse Cenpc1
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Cenpc1
Alternative Name:Mouse Centromere protein C 1, Cenpc1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Centromere protein C 1, Cenpc1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cenpc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Centromere protein C 1, Cenpc1 ELISA KIT has been pre-coated with an Mouse Centromere protein C 1, Cenpc1 antibody specific to Mouse Cenpc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cenpc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cenpc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cenpc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
protein and the Herpes simplex VP16 transactivator protein; C/A, CEN/ARS autonomous element for episomal replication in yeast; KanR, kanamycin resistance gene for selection in E. coli ; LEU2, LEU2 auxotrophic marker for selection in yeast; STE, N‐terminal
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
, add 50 µl of control (normal rabbit serum, mouse isotype control, etc.) to each tube and 25 µl of Protein G slurry (shake to suspend slurry before pipetting) and incubate for 1 h at 4 C. 5) Centrifuge of 1 min on microcentrifuge
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