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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Hyaluronidase PH-20, Spam1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Hyaluronidase PH- 20, SPAM1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Spam1,PH20,HEL-S-96n; HYA1; HYAL1; HYAL3; HYAL5; PH-20; PH20; SPAG15; epididymis secretory sperm binding protein Li 96n; hyal-PH20; hyaluronidase PH-20; hyaluronoglucosaminidase PH-20; sperm surface protein PH-20; sperm adhesion molecule
Search name
Mouse Spam1 ELISA KIT ,Mouse PH20 ELISA KIT ,Mouse HEL-S-96n ELISA KIT ,Mouse HYA1 ELISA KIT ,Mouse HYAL1 ELISA KIT ,Mouse HYAL3 ELISA KIT ,Mouse HYAL5 ELISA KIT ,Mouse PH-20 ELISA KIT ,Mouse PH20 ELISA KIT ,Mouse SPAG15 ELISA KIT ,Mouse epididymis secretory sperm binding protein Li 96n ELISA KIT ,Mouse hyal-PH20 ELISA KIT ,Mouse hyaluronidase PH-20 ELISA KIT ,Mouse hyaluronoglucosaminidase PH-20 ELISA KIT ,Mouse sperm surface protein PH-20 ELISA KIT ,Mouse sperm adhesion molecule ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Hyaluronidase PH- 20, SPAM1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronidase PH-20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hyaluronidase PH-20 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hyaluronidase PH-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hyaluronidase PH-20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
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All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
0.05% Tween-20 for 2 minutes, each time. 4) Proceed immediately to Signal Development, below. Signal Development 1) Dilute the 20X DAB Chromogen supplied with the kit in a sterile microcentrifuge tube just prior to use
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