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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Pre-mRNA-splicing regulator WTAP, Wtap含量
- 适应物种:
小鼠
- 标记物:
Pre-mRNA-splicing regulator WTAP, Wtap
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Pre-mRNA-splicing regulator WTAP, Wtap ELISA试剂盒
Product Name: Mouse Pre-mRNA-splicing regulator WTAP, Wtap ELISA试剂盒
Product Name: Mouse Pre-mRNA-splicing regulator WTAP, Wtap ELISA试剂盒
Gene Name: Mouse Pre-mRNA-splicing regulator WTAP, Wtap
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Pre-mRNA-splicing regulator WTAP, Wtap
Alternative Name: Mouse Pre-mRNA-splicing regulator WTAP, Wtap
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Pre-mRNA-splicing regulator WTAP, Wtap ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Pre-mRNA-splicing regulator WTAP, Wtap concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Pre-mRNA-splicing regulator WTAP, Wtap ELISA试剂盒 has been pre-coated with an Mouse Pre-mRNA-splicing regulator WTAP, Wtap antibody specific to Mouse Pre-mRNA-splicing regulator WTAP, Wtap .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pre-mRNA-splicing regulator WTAP, Wtap and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pre-mRNA-splicing regulator WTAP, Wtap , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pre-mRNA-splicing regulator WTAP, Wtap in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
at 200 x g for 5 min and aspirate wash. E. Repeat wash step of cell pellet. F. Pre-warm 6-well plates with 2 ml of complete medium per well at 37 °C. G. Add 2 μg of miRNA expression plasmid
Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
. 2003. Genome‐wide analysis of mRNA translation profiles in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 100:3889‐3894. Bentley, D.R., Balasubramanian, S., Swerdlow
Imaging of Endogenous RNA Using Genetically Encoded Probes
‐ATGATGTATGCTTTGTTTCT‐3′) and ND6R (5′‐CCTATTCCCCCGAGCAATCT‐3′) for mitochondrial ND6 mRNA, and ND1F (5′‐ATACCCATGGCCAACCTCCT‐3′) and ND1R (5′‐TTAGGTTTGAGGGGGAATGC‐3′) for controls
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