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LiF is a pleiotrophic factor produced by multiple cell types, including T cells, myelomonocytic lineages, fibroblasts, liver, heart and melanoma, Llf promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation, Other activities include the stimulation of acute phase protein synthesis by hepatocytes, stimulation of differentiation of cholinergic nerves, and suppression of adipogenesis by inhibiting the lipoprotein lipase in adipocytes. While humaniF is active on mouse cells and is widely used in the maintenance of murine ESC to prevent spontaneous differentiation, mouse LlF is not active on human cells due to its inability to bind to the human LIF receptor.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人白血病抑制因子 (LIF)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 LIF 单抗包被于酶标板上，标准品和样品中的 LIF与单抗结合，加入生物素化的抗人 LIF ，形成
Gene Knockout In Murine Embryonic Stem (ES) Cells
. Remove membrane from blotter neutralise in 2XSSC and place in oven to dry. Pre hybridise for at least 15 minutes in your favourite hybridisation solution and hybridise overnight with probe labelled with the Gigaprime kit. Separate
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