- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-16043m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Neurocan core protein, Ncan
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Neurocan core protein, Ncan ELISA KIT
Product Name:Mouse Neurocan core protein, Ncan ELISA KIT
Packing:96T
Catalog No.:ELI-16043m
Gene Name:Mouse Ncan
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Ncan
Alternative Name:Mouse Neurocan core protein, Ncan
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Neurocan core protein, Ncan ELISA KIT allows for the in vitro quantitative determination of Mouse Ncan concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Neurocan core protein, Ncan ELISA KIT has been pre-coated with an Mouse Neurocan core protein, Ncan antibody specific to Mouse Ncan .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ncan and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ncan , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ncan in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Transgenic Mouse and Transgenic Rat Outline
. Core personnel are available for consultation on all aspects of transgenic research. The transgenic founders are then bred to establish mouse lines and the offspring of each founder are tested for transgene expression. Contact Thom Saunders
for selection of stable clones. Alternative selection markers can be chosen from various antibiotic resistance genes or fluorescent markers such as enhanced green fluorescent protein (EGFP). Prepare plasmid DNA (~ 1 µg/µL) using a QIAGEN miniprep kit
Developing Genetically Engineered Mouse Models to Study Tumor Suppression
Costantini, F. and Lacy, E. 1981. Introduction of a rabbit beta‐globin gene into the mouse germ line. Nature 294:92‐94. Cui, X., Ji, D., Fisher, D.A., Wu, Y
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