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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
PRPF8 (Pre-mRNA-processing-splicing factor 8)
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse PRPF8 (Pre- mRNA- processing- splicing factor 8) ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
PRPF8 ELISA KIT,PRP8 ELISA KIT,PRP8 ELISA KIT,PRPC8 ELISA KIT,RP13 ELISA KIT,SNRNP220 ELISA KIT,220 kDa U5 snRNP-specific protein ELISA KIT,PRP8 homolog ELISA KIT,PRP8 pre-mRNA processing factor 8 homolog ELISA KIT,U5 snRNP-specific protein (220 kD) ELISA KIT, ortholog of S. cerevisiae Prp8p ELISA KIT,apoptosis-regulated protein 1 ELISA KIT,apoptosis-regulated protein 2 ELISA KIT,p220 ELISA KIT,precursor mRNA processing protein ELISA KIT,splicing factor Prp8 ELISA KIT,pre-mRNA processing factor 8 ELISA KIT
Intended use
Mouse PRPF8 ELISA kit allows for the in vitro quantitative determination of Mouse Pre- mRNA- processing- splicing factor 8 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Mouse PRPF8 ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRPF8, During the reaction, PRPF8 in the sample or standard competes with a fixed amount of biotin-labeled PRPF8 for sites on a pre-coated Monoclonal antibody specific to PRPF8. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PRPF8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
Order Mouse PRPF8 ELISA kit by email:
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文献和实验. First, the pre-crRNA array and tracrRNA are transcribed from theCRISPR locus. Second, tracrRNA hybridizes to the direct repeats of pre-crRNAand associates with Cas9 as a duplex, which mediates the processing of thepre-crRNA into mature crRNAs containing
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
NM_005381 Homo sapiens nucleolin (NCL), mRNA 2043 NM_005439 Homo sapiens myeloid leukemia factor 2 (MLF2), mRNA 697 NM_006445 Homo sapiens PRP8 pre-mRNA processing factor 8 homolog (yeast) (PRPF8), mRNA 1384 NM_019059 Homo sapiens homolog
Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
., Karbelashvili, M.S., Kirk, S.M., Li, H., Liu, X., Maisinger, K.S., Murray, L.J., Obradovic, B., Ost, T., Parkinson, M.L., Pratt, M.R., Rasolonjatovo, I.M., Reed, M.T., Rigatti, R., Rodighiero, C., Ross, M.T., Sabot, A., Sankar, S.V., Scally, A., Schroth, G.P
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