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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Choline O-acetyltransferase, Chat含量
- 适应物种:
小鼠
- 标记物:
Choline O-acetyltransferase, Chat
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Choline O- acetyltransferase, Chat ELISA KIT
Product Name:Mouse Choline O- acetyltransferase, Chat ELISA KIT
Packing:96T
Catalog No.:ELI-04658m
Gene Name:Mouse Chat
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Chat
Alternative Name:Mouse Choline O-acetyltransferase, Chat
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Choline O- acetyltransferase, Chat ELISA KIT allows for the in vitro quantitative determination of Mouse Chat concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Choline O- acetyltransferase, Chat ELISA KIT has been pre-coated with an Mouse Choline O-acetyltransferase, Chat antibody specific to Mouse Chat .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Chat and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Chat , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Chat in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
我发现了一个奇怪现象,U.E.Schambra(1989年)和他的同事在使用乙酰胆碱转移酶(choline acetyltransferase,ChAT)作为胆碱能神经元标志性蛋白追踪胆碱能神经元的时空定位时发现,在端脑背侧脑室区有许多正在进行有丝分裂的细胞表达ChAT。他们对此感到非常惊奇,又无法解释这种现象,故在论文中仍然把这些细胞看作胆碱能神经元(U.B.Schambra,1989年)。传统观点认为:已分化的功能神经元不可能进行有丝分裂。在端脑发育为大脑的过程中,其脑室区的神经上皮细胞分裂之后
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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