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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Laminin subunit beta-2, Lamb2含量
- 适应物种:
小鼠
- 标记物:
Laminin subunit beta-2, Lamb2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Laminin subunit beta- 2, Lamb2 ELISA KIT
Product Name:Mouse Laminin subunit beta- 2, Lamb2 ELISA KIT
Packing:96T
Catalog No.:ELI-04820m
Gene Name:Mouse Lamb2
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Lamb2
Alternative Name:Mouse Laminin subunit beta-2, Lamb2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Laminin subunit beta- 2, Lamb2 ELISA KIT allows for the in vitro quantitative determination of Mouse Lamb2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Laminin subunit beta- 2, Lamb2 ELISA KIT has been pre-coated with an Mouse Laminin subunit beta-2, Lamb2 antibody specific to Mouse Lamb2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lamb2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lamb2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lamb2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
secondary antibody review -- data from 99 publications
epithelial Caco2/bbe cells Bio-Rad 10 rhodamine immunocytochemistry 1:100 detect phospho-CREB in human colonic epithelial Caco2/bbe cells Bio-Rad 11 goat IgG FITC immunohistochemistry 1:40 detect ameloblastin in mouse postnatal mandibular molars
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Support Protocol 3: Mouse Euthanasia and Tissue Dissection Support Protocol 4: Serum Insulin Quantification Using ELISA Support Protocol 5: Analysis of Human Cell Engraftment by Flow Cytometry
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