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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Keratin, type II cytoskeletal 2 epidermal, Krt2含量
- 适应物种:
小鼠
- 标记物:
Keratin, type II cytoskeletal 2 epidermal, Krt2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 ELISA试剂盒
Product Name: Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 ELISA试剂盒
Product Name: Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 ELISA试剂盒
Gene Name: Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2
Alternative Name: Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 ELISA试剂盒 has been pre-coated with an Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 antibody specific to Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Keratin, type II cytoskeletal 2 epidermal, Krt2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
., Metzger, D., Chambon, P., Rajewsky, K., Krieg, T., Haase, I., and Pasparakis, M. 2006. Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling. Hum. Mol. Genet
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
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