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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pCMV-SPORT6-MTPN(NM_145808.3)
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pCMV-SPORT6-MTPN(NM_145808.3)/ pCMV-SPORT6-MTPN(NM_145808.3)璐ㄧ矑
pCMV-SPORT6-MTPN(NM_145808.3) Plasmid
PVT16604 2ug
pCMV-SPORT6-MTPN(NM_145808.3) Plasmid Alternative Name
NM_145808.3,GCDP, V-1
pCMV-SPORT6-MTPN(NM_145808.3) PlasmidInformation
Promoter
Prokaryotic resistance Amp
Screening marker
Clone strain DH5a
Culture Conditions LB/37 Celsius degree
Forward primer SP6
Reverse primer T7
pCMV-SPORT6-MTPN(NM_145808.3) Plasmid Description
pCMV-SPORT6-MTPN(NM_145808.3) Plasmid is a DNA cloning plasmid
NOTE: For research use only!
3128136151
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文献和实验检测:质粒电泳一般有三条带,分别为质粒的超螺旋、开环、线型三种构型 吸光值检测:采用分光光度计检测260nm、280nm波长吸光值,若吸光值260nm/280nm的比值介于1.7-1.9之间,说明质粒质量较好,1.8为最佳,低于1.8说明有蛋白质污染,大于1.8说明有RNA污染。
in 0.4 ml of TE buffer (10 mM Tris-Cl, 1 mM EDTA, pH 7.5). Add 10ul of RNAse A solution (20 mg/ml stock stored at -20 ℃), vortex and incubate at 37 ℃ for 20 to 30 minutes to digest remaining RNA. 8. Extract proteins from the plasmid DNA using PCIA
and long term storage. 17. Measure the concentration of the plasmid DNA by diluting stock into water at 1:200 or 5 m l of DNA per 1 ml of water (blank spectrophotometer to water). The absorbance at 260 nm multiplied by ten is the concentration
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