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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pEGFP-Tnfsf10-m(39-291bp)
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pEGFP-Tnfsf10-m(39-291bp)/ pEGFP-Tnfsf10-m(39-291bp)璐ㄧ矑
pEGFP-Tnfsf10-m(39-291bp)
PVT13734 2ug
pEGFP-Tnfsf10-m(39-291bp) Search name
pEGFP-Tnfsf10-m(39-291bp),pEGFP-Tnfsf10-m(39-291bp) vector,pEGFP-Tnfsf10-m(39-291bp) plasmid,pEGFP-Tnfsf10-m(39-291bp) map
pEGFP-Tnfsf10-m(39-291bp) Information
Bacterial Resistance(s): Kan
Screening: G418
Growth Strain(s): DH5 alpha
Growth Condition: LB/37 Centigrade
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文献和实验Method for Single-Cell Electroporation
Laboratories, Palo Alto, CA) plasmid pEGFP, which drives green fluorescent protein expression (GFP) with a strong CMV promoter. Single cells transfected with pEGFP expressed bright GFP within 12 h after electroporation, detectable by epifluorescence
with insertion of annealed sgRNA oligos will show only linearized plasmid,whereas clones without insertion will yield two fragments, withsizes of ~980 and ~7,520 bp in the case of pX330 when runningand visualizing on an agarose gel. 7. Sequencing verifi
encoding enhanced GFP (pEGFP-C1; Clontech) together with 1 µg of either a control plasmid (pMEX), or a pCMV plasmid carrying either the pro-apoptotic adenovirus E1A or the anti-apoptotic adenovirus E1B-19K gene (20 ,21 ) using 10 µl LipofectAMINE Reagent
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