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- LSM BIO
- 进口
- PVT11529
- 2025年07月13日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pZSm45 ndhII
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pZSm45 ndhII/ pZSm45 ndhII璐ㄧ矑
pZSm45 ndhII
Catalog No.PVT11529
Packing 2ug
pZSm45 ndhII Alternative name
pZSm45 ndhIIpZSm45 ndhII vector, pZSm45 ndhII plasmid, pZSm45 ndhII map, pZSm45 ndhII sequence,
Promoter -
Replicon -
Terminator -
Resistance -
Strain -
Culture conditions -
Forward primer -
Reverse primer -
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文献和实验This is a standard large scale prep.for plasmid DNA which gives a yield of 0.5-1.0mg.I have made some minor changes to the MHB protocol. Solutions Solution I,II,III from protocol D.1. Tris/EDTA pH 7.5 (optional) 20 ml 1 M Tris 7.5 4 ml
PREPARATION OF PLASMID DNA FOR SEQUENCING
. Incubate at 37℃ overnight with vigorous shaking. Follow the Plasmid Quick Prep protocol through the potassium acetate step to isolate plasmid DNA. Add DNAse-free RNAse to 50µg/ml, incubate, 37℃ from 10-30 minutes. Phenol extract the solution, saving
High Efficency Yeast Plasmid Rescue
Apply supernatants to QIAprep spin column. Centrifuge column for 45 seconds on full blast. Discard flow-though. Wash column with 750 µl of Buffer PE and centrifuge 45 seconds. Discard flow through and spin for an additional
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