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- LSM BIO
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- PVT10833
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pHes1 (467)-luc
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pHes1 (467)-luc/ pHes1 (467)-luc璐ㄧ矑
pHes1(467)-luc
Catalog No. PVT10833
Packing 2ug
Function Mammal reporter plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer
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文献和实验Plasmid DNA Isolation from Bacteria
broth. Transfer 1.5ml to a micro centrifuge tube and spin 2min. Decant the supernatant. Repeat with another 1.5ml of cells. Drain well onto a Kimwipe. 2.Resuspend the pellet in 467μl TE buffer by repeated pipetting. Add 30μl of 10% SDS and 3μl of 20
HeTube Mating with MSCV recipient
8 ml of 50% Glucose (filter sterile) Strain and vector information: Recipient pMSCV: PheS gene flanked with 2-Isce-I site and homologous sites for recombination, 10.6 kb. Carbenicillin (amp) resistent. Recipient Strain BW28705I/pML300: pML300
He Tube Mating with MSCV recipient
(filter sterile) Strain and vector information: Recipient pMSCV: PheS gene flanked with 2-Isce-I site and homologous sites for recombination, 10.6 kb. Carbenicillin (amp) resistent. Recipient Strain BW28705I/pML300: pML300 plasmid carries
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