- ￥1080
- LSM BIO
- 进口
- PVT1302
- 2026年05月21日
8 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
相关阅读
细胞污染识别与处理全攻略：5 种常见类型+关键误区Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
Fop-Flash
- 库存：
10
- 供应商：
LSMBIO
- 规格：
质粒干粉
Fop-Flash
Search name
Fop-Flash,Plasmid Fop-Flash,Fop-Flash vector
Fop-Flash Information
Promoter: TK
Replicon: pUC ori
Terminator: SV40 poly (A) signal
Plasmid classification: mammalian cells, signal pathway reporting vectors
Prokaryotic resistance: ampicillin Amp
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 37 centigrade, aerobic, LB
Expression host: mammalian cells
5'sequencing primers: primers designed according to sequence
3'sequencing primers: primers designed according to sequence
Fop-Flash Sequence
LOCUS Exported 5529 bp ds-DNA circular SYN 24-APR-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5529
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 590..652
/note="Mini-TK promoter"
/note="minimal herpes simplex virus (HSV) thymidine kinase
promoter"
CDS 681..2333
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/note="luciferase"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 2492..2557
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
CDS 2687..2707
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
polyA_signal 3132..3266
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3710..4298)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4469..5329)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5330..5434)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa agggggtaag gccaaagggg
421 gtaaggccaa agggggcccc ctttggcctt accccctttg gccttacccc ctttggcctt
481 acctgcatgc ctgcaggtcg actctagagg atccggcccc gcccagcgtc ttgtcattgg
541 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
601 aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt aacagcgtca
661 acagcgtgcc gcagatctcc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
721 tctatcctct agaggatgga accgctggag agcaactgca taaggctatg aagagatacg
781 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg
841 cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
901 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
961 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
1021 tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc
1081 aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt
1141 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
1201 ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac
1261 tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa
1321 ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
1381 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
1441 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt
1501 tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat
1561 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
1621 ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc
1681 cagggatacg acaaggatat gggctcactg agactacatc agctattctg attacacccg
1741 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
1801 tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag
1861 gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
1921 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
1981 tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat
2041 tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg
2101 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
2161 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
2221 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
2281 aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg taaaatgtaa
2341 ctgtattcag cgatgacgaa attcttagct attgtaatga attcgagctc caccgcggtg
2401 gcggccgctc tagaggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
2461 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
2521 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
2581 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
2641 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验该产品被引用文献
Structural basis for the interaction of the chaperone Cbp3 with newly synthesized cytochrome b during mitochondrial respiratory chain assembly
Mama Ndi1, Geoffrey Masuyer1, Hannah Dawitz1, Andreas Carlstr?m1, Mirco Michel1, Arne Elofsson2, Mikaela Rapp1, P?l Stenmark3 and Martin Ott3*
First Published on Journal of biological chemistry
September 19, 2019
doi: 10.1074/jbc.RA119.010483
jbc.RA119.010483.
相关实验
Protein Labeling With FlAsH and ReAsH
. The recently developed Fluorescein Arsenical Hairpin (FlAsH/tetracysteine) binder technology emerged as a promising alternative to FP for protein labeling and cellular localization studies. The combination of a small genetically encoded peptide tag with a small
How does RNAi work? Genetic and biochemical data indicate a possible two-step mechanism for RNA interference (RNAi): an initiation step and an effector step. a | In the first step, input double-stranded (ds
1978年，Sill发明了FLASH色谱，文章发表在J.O.C上，从此FLASH色谱方法得以建立，新的设备也不断涌现，在国内，大连理工大学的彭勤纪教授自国外归国后，引进了该技术并进行了系统综述，发表在《化工进展》上，该文章首次将FLASH色谱译为“快速色谱”。狭义的快速色谱是指用压缩气体产生压力，加速流动相洗脱速度的低压短柱制备液相色谱系统，广义的快速色谱系统是指一切输液方式的中低压液相色谱系统。快速色谱有人还译为“闪式色谱”“FLASH色谱”。 经过20余年的发展
技术资料暂无技术资料 索取技术资料
文献支持
Fop-Flash Plasmid
￥1080
