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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pMG36e
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pMG36e
PVT5201 2ug
Search name
pMG36e,Plasmid pMG36e,pMG36e vector
pMG36e Plasmid Information
Promoter: P32
Replicator: pWV01 ori
Terminator: T
Plasmid classification: Lactococcus lactis constitutively expressed plasmid
Plasmid size: 3610bp
Resistance: Erythromycin
Tag: Erythromycin
Clone strain: DH5 alpha
Culture conditions: 37 DEG C, aerobic, LB
Host: Lactococcus lactis
Induction mode: no need to induce
Primers for 5'sequencing: Based on sequence design
Primers for 3'sequencing: Based on sequence design
Expression: Bacterial
Use:Lactic Acid Bacteria Plasmids
pMG36e Plasmid Sequence
LOCUS Exported 3610 bp ds-DNA circular SYN 14-JUL-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pMG36e
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3610)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, July 14, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..3610
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 227..925
/codon_start=1
/note="repA"
/translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
VLDAETGEIK"
CDS complement(1463..2197)
/codon_start=1
/note="Emr"
/translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
DLNNISFEQFLSLFNSYKLFNK"
misc_feature 2474..2652
/note="P32"
CDS 2653..2892
/codon_start=1
/note="ORF"
/translation="MAIVSAEKFVIRARPGIDPLESTCRHASLQSLKTKVAAAVEAAKT
VGKGDGTTGTSDKGGGQGTPALRYLELRFKVKWY"
CDS complement(3215..3226)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
ORIGIN
1 ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc aagataaaaa
61 attaacagat atggcgaaac aaaaaggttt ttcaaaatct gcggttgcgg cgttagctat
121 agaagaatat gcaagaaagg aatcagaaca aaaaaaataa gcgaaagctc gcgtttttag
181 aaggatacga gttttcgcta cttgtttttg ataaggtaat tatatcatgg ctattaaaaa
241 tactaaagct agaaattttg gatttttatt atatcctgac tcaattccta atgattggaa
301 agaaaaatta gagagtttgg gcgtatctat ggctgtcagt cctttacacg atatggacga
361 aaaaaaagat aaagatacat ggaatagtag tgatgttata cgaaatggaa agcactataa
421 aaaaccacac tatcacgtta tatatattgc acgaaatcct gtaacaatag aaagcgttag
481 gaacaagatt aagcgaaaat tggggaatag ttcagttgct catgttgaga tacttgatta
541 tatcaaaggt tcatatgaat atttgactca tgaatcaaag gacgctattg ctaagaataa
601 acatatatac gacaaaaaag atattttgaa cattaatgat tttgatattg accgctatat
661 aacacttgat gaaagccaaa aaagagaatt gaagaattta cttttagata tagtggatga
721 ctataatttg gtaaatacaa aagatttaat ggcttttatt cgccttaggg gagcggagtt
781 tggaatttta aatacgaatg atgtaaaaga tattgtttca acaaactcta gcgcctttag
841 attatggttt gagggcaatt atcagtgtgg atatagagca agttatgcaa aggttcttga
901 tgctgaaacg ggggaaataa aatgacaaac aaagaaaaag agttatttgc tgaaaatgag
961 gaattaaaaa aagaaattaa ggacttaaaa gagcgtattg aaagatacag agaaatggaa
1021 gttgaattaa gtacaacaat agatttattg agaggaggga ttattgaata aataaaagcc
1081 ccctgacgaa agtcgaaggg ggtttttatt ttggtttgat gttgcgatta atagcaatac
1141 aattgcaata aacaaaatga tcgacctcgg gacccctatc tagcgaactt ttagaaaaga
1201 tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta gatgtctaaa
1261 aagctagctt tttagacatc taaatctagg tactaaaaca attcatccag taaaatataa
1321 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
1381 tgttcttccc cgatcgaccc gattcacaaa aaataggcac acgaaaaaca agttaaggga
1441 tgcagtttat gcatccctta acttacttat taaataattt atagctattg aaaagagata
1501 agaattgttc aaagctaata ttgtttaaat cgtcaattcc tgcatgtttt aaggaattgt
1561 taaattgatt ttttgtaaat attttcttgt attctttgtt aacccatttc ataacgaaat
1621 aattatactt ttgtttatct ttgtgtgata ttcttgattt ttttctactt aatctgataa
1681 gtgagctatt cactttaggt ttaggatgaa aatattctct tggaaccata cttaatatag
1741 aaatatcaac ttctgccatt aaaagtaatg ccaatgagcg ttttgtattt aataatcttt
1801 tagcaaaccc gtattccacg attaaataaa tctcattagc tatactatca aaaacaattt
1861 tgcgtattat atccgtactt atgttataag gtatattacc atatatttta taggattggt
1921 ttttaggaaa tttaaactgc aatatatcct tgtttaaaac ttggaaatta tcgtgatcaa
1981 caagtttatt ttctgtagtt ttgcataatt tatggtctat ttcaatggca gttacgaaat
2041 tacacctctt tactaattca agggtaaaat ggccttttcc tgagccgatt tcaaagatat
2101 tatcatgttc atttaatctt atatttgtca ttattttatc tatattatgt tttgaagtaa
2161 taaagttttg actgtgtttt atatttttct cgttcattat aaccctcttt aatttggtta
2221 tatgaatttt gcttattaac gattcattat aaccacttat tttttgtttg gttgataatg
2281 aactgtgctg attacaaaaa tactaaaaat gcccatattt tttcctcctt ataaaattag
2341 tataattata gcacggtcga tcttctatat aaaagatata ttatcttatc agtattgtca
2401 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
2461 aaatatgggt cgatcgaatt cggtcctcgg gatatgataa gattaatagt tttagctatt
2521 aatctttttt tatttttatt taagaatggc ttaataaagc ggttactttg gatttttgtg
2581 agcttggact agaaaaaaac ttcacaaaat gctatactag gtaggtaaaa aaatattcgg
2641 aggaattttg aaatggcaat cgtttcagca gaaaaattcg taattcgagc tcgcccgggg
2701 atcgatcctc tagagtcgac ctgcaggcat gcaagcttgc aaagtctgaa aacgaaggtg
2761 gcagctgccg ttgaagcggc caagacagtt ggtaaaggcg acggtacaac cggtactagc
2821 gacaaaggcg gcggtcaagg taccccggcg ctacgatatt tggagttgag gttcaaagtc
2881 aaatggtact gatgaccggt aaaatttaat attttgaacc ttgcttaggc agctgacttc
2941 acattgttga gatcagctgc cttttgctta tagttcattg agtagaaacg gttctgttgc
3001 gaagtttgaa aatcaaacgc aagctcgatt ttttattaaa acgtctcaaa atcgtttctg
3061 agacgtttta gcgtttattt cgtttagtta tcggcataat cgttaaaaca ggcgttatcg
3121 tagcgtaaaa gcccttgagc gtagcgtggc tttgcagcga agatgttgtc tgttagatta
3181 tgaaagccga tgactgaatg aaataataag cgcagcgccc ttctatttcg gttggaggag
3241 gctcaaggga gtatgaggga atgaaattcc ctcatgggtt tgattttaaa aattgcttgc
3301 aattttgccg agcggtagcg ctggaaaatt tttgaaaaaa atttggaatt tggaaaaaaa
3361 tggggggaaa ggaagcgaat tttgcttccg tactacgacc ccccattaag tgccgagtgc
3421 caatttttgt gccaaaaacg ctctatccca actggctcaa gggtttaagg ggtttttcaa
3481 tcgccaacga atcgccaacg ttttcgccaa cgttttttat aaatctatat ttaagtagct
3541 ttattgttgt ttttatgatt acaaagtgat acactaactt tataaaatta tttgattgga
3601 gttttttaat
//
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文献和实验该产品被引用文献
Structural basis for the interaction of the chaperone Cbp3 with newly synthesized cytochrome b during mitochondrial respiratory chain assembly
Mama Ndi1, Geoffrey Masuyer1, Hannah Dawitz1, Andreas Carlstr?m1, Mirco Michel1, Arne Elofsson2, Mikaela Rapp1, P?l Stenmark3 and Martin Ott3*
First Published on Journal of biological chemistry
September 19, 2019
doi: 10.1074/jbc.RA119.010483
jbc.RA119.010483.
相关实验
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
Plasmid Profile Typing and Plasmid Fingerprinting
Plasmids are extra-chromosomal molecules of deoxyribonucleic acid (DNA) capable of autonomous replication. Such molecules have been identified in many bacterial genera and usually exist as covalently closed circular (CCC) molecules. Plasmids
质粒 plasmid 在细胞内整个世代稳定地传递给后代，但并不在染色体上而进行自主增殖的一类遗传因子的总称。该类因子的存在与否，对细胞的生存无关紧要。这个名词主要指细菌细胞内的因子，它们包括可在细胞间发生传递的F因子、 R因子、大肠杆菌素因子等。此外，还有各种本身不能传递的因子。在具有质粒细胞的后代中，有时会出现低频率的失去质粒的细菌。在质粒中，对那些能变成插入奇主染色体状态的特殊质粒，特称为附加体（episome）。
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pMG36e Plasmid
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