S17-1 lamp pir 感受态细胞(化转)/ S17-

S17-1 LAMP PIR Chemically Competent Cell

Packing

S17-1                        10 x 0.1 ml/tube

pUC19 (control vector,10pg/μl)   10ul

Store at –80°C

 

Genotype

RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510

Alternative name
S17-1 LAMP PIR chemically Competent Cells,S17-1 LAMP PIR Competent E.coli,S17-1 LAMP PIR Competent Cells, S17-1 LAMP PIR chemically E.coli Express Competent Cells 

Transformation Protocol

1.Please storage S17-1 LAMP PIR Chemically Competent Cell in -80°C for late use.

 

2. Thaw a tube of S17-1 LAMP PIR Chemically Competent Cell锛?00μl 锛塷n ice.

 

3. Add 1µl plasmid DNA or 10µl ligation product to the cell mixture. Carefully flick the tube 4–5 times to mix cells and DNA. Do not vortex.

 

4.Place the mixture on ice for 25 minutes. Do not mix.

 

5. Heat shock at exactly 42°C for exactly 45 seconds and then immediately transfer the tube back into ice for 2min. Do not mix.

 

6. Add 700ul LB then put on the Constant temperature shaker (200 rpm) for 45min,Warm selection plates to 37°C.

 

8. Pellet the mixture by centrifugation at 5000rpm/min for 3min. Pour off about 100ul of cell supernatant, then resuspend the cell pellet in the remaining medium by gently vortexing the tube.

 

9.Spread the cell on the agar plates by antibiotic, then place it on 37°C for 1hour.

 

10. Incubate overnight at 37°C.

 

 

Caution:

 

1.STORAGE AND HANDLING: Competent cells should be stored at–80°C. Storage at –20°C will result in a significant decrease in transformation efficiency. Cells lose efficiency whenever they are warmed above –80°C, even if they do not thaw.

 

2. This product is FOR RESEARCH USE ONLY!