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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Casein Kinase 1 epsilon
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
Casein Kinase 1 epsilon ELISA KIT|Human
Catalog EF008396
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
Casein Kinase 1 epsilon ELISA KIT Sensitivity< 0.188ng/ml
Application Casein Kinase 1 epsilon ELISA KIT|Human is for detecting the concentration of Human Casein Kinase 1 epsilon in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of Casein Kinase 1 epsilon ELISA KIT|Human
The microtiter plate provided in Casein Kinase 1 epsilon ELISA KIT|Human has been pre-coated with an Human Casein Kinase 1 epsilon antibody specific to Human Casein Kinase 1 epsilon. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human Casein Kinase 1 epsilonantibody preparation specific for Human Casein Kinase 1 epsilon then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Casein Kinase 1 epsilon, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human Casein Kinase 1 epsilonin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that Casein Kinase 1 epsilon ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S. 2002b. The protein kinase complement of the human genome. Science 298:1912‐1934.
-6 Casein Kinase I epsilon and delta (CKI-epsilon and CKI-delta) (17) 520-538 AACUGGGGAAGAAGGGCAACC HEK
AAGGGAAAGAAGGAGGUUUCA RBL-2H3, WEHI-231, NALM-6 Bruton's tyrosine kinase (Btk) (33) NM_000061 518-536 AAGAAGCUUAAAACCUGGGAG RBL-2H3, WEHI-231, NALM-6 Casein Kinase I epsilon and delta (CKI-epsilon and CKI-delta) (17) 520-538 AACUGGGGAAGAAGGGCAACC HEK293 Caspase 1 (34
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