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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Multidrug resistance-associated protein 4,ABCC4 ELISA KI
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Multidrug resistance-associated protein 4, ABCC4 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ABCC4,MRP4,MOAT-B; MOATB; MRP/cMOAT-related ABC transporter; ATP-binding cassette, sub-family C (CFTR/MRP) member 4; canalicular multispecific organic anion transporter (ABC superfamily); multi-specific organic anion transporter B; ATP-binding cassette sub-family C (CFTR/MRP) member 4,Multi-specific organic anion transporter B,ATP-binding cassette sub-family C member 4,MRP/cMOAT-related ABC transporter, Multidrug resistance-associated protein 4
Search name
Human ABCC4 ELISA KIT ,Human MRP4 ELISA KIT ,Human MOAT-B ELISA KIT ,Human MOATB ELISA KIT ,Human MRP/cMOAT-related ABC transporter ELISA KIT ,Human ATP-binding cassette ELISA KIT ,Human sub-family C (CFTR/MRP) member 4 ELISA KIT ,Human canalicular multispecific organic anion transporter (ABC superfamily) ELISA KIT ,Human multi-specific organic anion transporter B ELISA KIT ,Human ATP-binding cassette sub-family C (CFTR/MRP) member 4 ELISA KIT ,Human Multi-specific organic anion transporter B ELISA KIT ,Human ATP-binding cassette sub-family C member 4 ELISA KIT ,Human MRP/cMOAT-related ABC transporter ELISA KIT ,Human Multidrug resistance-associated protein 4
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human multidrug resistance-associated protein 4,Multidrug resistance-associated protein 4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Multidrug resistance-associated protein 4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Multidrug resistance-associated protein 4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Multidrug resistance-associated protein 4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Multidrug resistance-associated protein 4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Flow Cytometric Evaluation of Multidrug Resistance Proteins
cells is important for this reason. Here, we review literature on flow cytometric detection of the three most studied transporters: P-glycoprotein, multidrug resistance-associated proteins, and breast cancer resistance protein.
-67 antigen. ref: 1. Gerdes J, Schwab U, Lemke H, Stein H. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int J Cancer 31:13–20. 2. The Ki-67 Protein
Impact of Breast Cancer Resistance Protein on Cancer Treatment Outcomes
Breast cancer resistance protein (BCRP/ABCG2) was discovered in multidrug resistant breast cancer cells having an ATP-dependent transport-based resistance phenotype. This ABC transporter functions (at least in part) as a xenobiotic protective
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