- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-30335h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human GTPase KRas,KRAS
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human GTPase KRas, KRAS ELISA KIT
Product Name:Human GTPase KRas, KRAS ELISA KIT
Packing:96T
Catalog No.:ELI-30335h
Gene Name:KRAS
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:GTPase KRas(KRAS
Alternative Name:KRAS,GTPase KRas(KRAS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human GTPase KRas, KRAS ELISA KIT allows for the in vitro quantitative determination of GTPase KRas(KRAS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human GTPase KRas, KRAS ELISA KIT has been pre-coated with an GTPase KRas(KRAS antibody specific to GTPase KRas(KRAS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GTPase KRas(KRAS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GTPase KRas(KRAS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of GTPase KRas(KRAS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
) OncoCarta™ Panel 1.0 19 ABL1 (14); AKT1 (7); AKT2 (2); BRAF (28); CDK4 (2);EGFR (40); ERBB2 (8); FGFR1 (2); FGFR3 (9); FLT3(3); HRAS (12); JAK2 (1); KIT (32); KRAS (18); MET(5); NRAS (18); PDGFRA (11
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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