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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Blood group Rh(CE) polypeptide,RHCE
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Blood group Rh(CE) polypeptide, RHCE ELISA KIT
Product Name:Human Blood group Rh(CE) polypeptide, RHCE ELISA KIT
Packing:96T
Catalog No.:ELI-42747h
Gene Name:RHCE
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Blood group Rh(CE) polypeptide(RHCE
Alternative Name:RHCE,Blood group Rh(CE) polypeptide(RHCE
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Blood group Rh(CE) polypeptide, RHCE ELISA KIT allows for the in vitro quantitative determination of Blood group Rh(CE) polypeptide(RHCE concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Blood group Rh(CE) polypeptide, RHCE ELISA KIT has been pre-coated with an Blood group Rh(CE) polypeptide(RHCE antibody specific to Blood group Rh(CE) polypeptide(RHCE .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Blood group Rh(CE) polypeptide(RHCE and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Blood group Rh(CE) polypeptide(RHCE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Blood group Rh(CE) polypeptide(RHCE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验PCRELISA for High-Throughput Blood Group Genotyping
minor blood group antigens were adapted to this method and are described in this work: Rh (D, C, c, E, e), Kell (K, k), Duffy (Fya , Fyb ), and Kidd (Jka , Jkb ). Other blood group antigens could be easily tested this way as long as their molecular basis
with anti�E (61.70%), 8 with anti�D (17.02%), anti�cE 5(10.64%), 4 with anti�c (8.51%) and 1 with anti�C (2.13%). All the 47 Rh blood group antibodies were IgG or IgG+IgM, and were reactive to red blood cells with corresponding antigens at 37℃
下面是我设计的引物的BLAST结果,请大虾帮我解释一下。谢谢
rhesus D ca... 36 3.4 gi|999309|gb|S78509.1|S78509 RhD [human, D--phenotype, eryt... 36 3.4 gi|693866|gb|S73913.1|S73913 Rh D=RhK562-II [human, erythro... 36 3.4 gi|546725|gb|S70174.1|S70174 RhD=Rh blood group system D-an... 36 3.4 gi|299051|gb|S57971
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