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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Blood group Rh(D) polypeptide,RHD
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Blood group Rh(D) polypeptide, RHD ELISA KIT
Product Name:Human Blood group Rh(D) polypeptide, RHD ELISA KIT
Packing:96T
Catalog No.:ELI-30319h
Gene Name:RHD
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Blood group Rh(D) polypeptide(RHD
Alternative Name:RHD,Blood group Rh(D) polypeptide(RHD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Blood group Rh(D) polypeptide, RHD ELISA KIT allows for the in vitro quantitative determination of Blood group Rh(D) polypeptide(RHD concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Blood group Rh(D) polypeptide, RHD ELISA KIT has been pre-coated with an Blood group Rh(D) polypeptide(RHD antibody specific to Blood group Rh(D) polypeptide(RHD .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Blood group Rh(D) polypeptide(RHD and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Blood group Rh(D) polypeptide(RHD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Blood group Rh(D) polypeptide(RHD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验血型 blood group 将不同个体的血液混合时,红细胞中的凝集原与另一个体血清中的凝集素发生作用,有时可出现凝集。由于凝集只在一定组合的条件下发生,因此接凝集的有无而将血液分为若干类型,这种类型称为血型。即属于同一血型者不凝集,如果发生凝集时则说明血型不同。在人类根据红细胞的抗原物质的特性,可分为 ABO血型或者 Rh血型等若干个类型。 ABO血型和 Rh血型在输血上有重要意义。对人类来说,最初就是根据 ABO血型而知道了凝集原与凝集素的关系,并阐明是通过复对立基
The Use of Maternal Plasma for Prenatal RhD Blood Group Genotyping
Alloimmunization to the blood group antibody anti-RhD (anti-D) is the most common cause of hemolytic disease of the fetus and newborn. Knowledge of fetal D type in women with anti-D makes management of the pregnancy much easier and avoids
PCRELISA for High-Throughput Blood Group Genotyping
minor blood group antigens were adapted to this method and are described in this work: Rh (D, C, c, E, e), Kell (K, k), Duffy (Fya , Fyb ), and Kidd (Jka , Jkb ). Other blood group antigens could be easily tested this way as long as their molecular basis
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