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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.312-20ng/ml
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Hydroxyacyl-coenzyme A dehydrogenase mitochondrial,HADH
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human Hydroxyacyl- coenzyme A dehydrogenase mitochondrial, HADH ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HADH,HAD; HADH1; HADHSC; HCDH; HHF4; MSCHAD; SCHAD; L-3-hydroxyacyl-Coenzyme A dehydrogenase, short chain; hydroxyacyl-coenzyme A dehydrogenase, mitochondrial; medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase; short-chain 3-hydroxyacyl-CoA dehydrogenase; hydroxyacyl-CoA dehydrogenase ,Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial,Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase,Short-chain 3-hydroxyacyl-CoA dehydrogenase,
Search name
Human HADH ELISA KIT ,Human HAD ELISA KIT ,Human HADH1 ELISA KIT ,Human HADHSC ELISA KIT ,Human HCDH ELISA KIT ,Human HHF4 ELISA KIT ,Human MSCHAD ELISA KIT ,Human SCHAD ELISA KIT ,Human L-3-hydroxyacyl-Coenzyme A dehydrogenase ELISA KIT ,Human short chain ELISA KIT ,Human hydroxyacyl-coenzyme A dehydrogenase ELISA KIT ,Human mitochondrial ELISA KIT ,Human medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase ELISA KIT ,Human short-chain 3-hydroxyacyl-CoA dehydrogenase ELISA KIT ,Human hydroxyacyl-CoA dehydrogenase ELISA KIT ,Human Hydroxyacyl-coenzyme A dehydrogenase ELISA KIT ,Human mitochondrial ELISA KIT ,Human Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase ELISA KIT ,Human Short-chain 3-hydroxyacyl-CoA dehydrogenase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial, HCDH concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HCDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HCDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HCDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HCDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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