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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Chemokine- binding protein 2, CCBP2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Chemokine- binding protein 2, CCBP2 ELISA KIT
Product Name:Human Chemokine- binding protein 2, CCBP2 ELISA KIT
Packing:96T
Catalog No.:ELI-11368h
Gene Name:CCBP2
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Chemokine-binding protein 2(CCBP2
Alternative Name:CCBP2,Chemokine-binding protein 2(CCBP2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Chemokine- binding protein 2, CCBP2 ELISA KIT allows for the in vitro quantitative determination of Chemokine-binding protein 2(CCBP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Chemokine- binding protein 2, CCBP2 ELISA KIT has been pre-coated with an Chemokine-binding protein 2(CCBP2 antibody specific to Chemokine-binding protein 2(CCBP2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Chemokine-binding protein 2(CCBP2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Chemokine-binding protein 2(CCBP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Chemokine-binding protein 2(CCBP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Characterization of Chemokine Receptors
and equipment for Bradford protein assay ( appendix 3A ) Alternate Protocol 1: SPA‐Based [35S]GTPγS Binding to Cell Membranes Expressing Chemokine Receptors
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Analysis of Protein‐DNA Binding Sites Basic Protocol 2: Analysis of PCR Array Data Support Protocol 1: Preparation of Annealed Linkers Reagents and Solutions Commentary
our extensive experience with B-PER for isolating fusion proteins in which the fusion partner is either IPIII-His (an internal protein of bacteriophage T4 with a His tag at the C-terminus)or MBP (the E.coli maltose binding protein).The two cases illustrate
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