Human Sphingosine 1 Phosphate

Human Sphingosine 1 Phosphate Receptor 2, S1PR2 ELISA kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Sphingosine 1-phosphate receptor 2(S1P receptor 2,S1P2),;

Endothelial differentiation G-protein coupled receptor 5,Sphingosine 1-phosphate receptor Edg-5(S1P receptor Edg-5),

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of human sphingosine 1-phosphate receptor 2,S1P receptor 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P receptor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for S1P receptor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain S1P receptor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S1P receptor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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