- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELA-E9909h
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Sphingosine 1 Phosphate Receptor 1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Sphingosine 1- phosphate receptor 1, S1PR1 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Sphingosine 1-phosphate receptor 1(S1P receptor 1,S1P1),;
Endothelial differentiation G-protein coupled receptor 1,Sphingosine 1-phosphate receptor Edg-1(S1P receptor Edg-1),;
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of human sphingosine 1-phosphate receptor 1,S1P receptor 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S1P receptor 1, During the reaction, S1P receptor 1 in the sample or standard competes with a fixed amount of biotin-labeled S1P receptor 1 for sites on a pre-coated Monoclonal antibody specific to S1P receptor 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of S1P receptor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Measuring the effects of sphingosine-1-phosphate (S1P) receptor modulators on human primary neural cells is of particular interest given the recent application of these central nervous system-accessible agents to the treatment
Capture and Qualitative Analysis of the Activated Fc Receptor Complex from Live Cells
the isolation of activated Fc receptor complexes from RAW 264.7 macrophages using live?cell affinity receptor chromatography (LARC). The Fc receptor complex is activated and captured by IgG?coated microbeads on the surface of live macrophages. After the cells
有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
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