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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
free fatty acids
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/ml
- 规格:
96T
Human free fatty acids ELISA Kit
Product Name:Human free fatty acids ELISA Kit
Packing:96T
Catalog No.:ELA-E0612h
Gene Name:FFA
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:free fatty acids
Alternative Name:FFA,free fatty acids
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human free fatty acids ELISA Kit allows for the in vitro quantitative determination of free fatty acids concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human free fatty acids ELISA Kit has been pre-coated with an free fatty acids antibody specific to free fatty acids .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for free fatty acids and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain free fatty acids, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of free fatty acids in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Glucuronidation of Fatty Acids and Prostaglandins by Human UDP-Glucuronosyltransferases
This chapter summarizes methods and experimental approaches involved in the study of glucuronidation of several naturally occurring metabolites of the free fatty acids (FFAs), linoleic acid (LA), and arachidonic acid (AA). Data on FA
Analysis of Fatty Acids by Gas Liquid Chromatography
The fatty acids (FA) of animal, plant, and microbial origin are predominantly unbranched aliphatic chains with an even number of carbon atoms and a single carboxyl group. FA can be classified as saturated, monounsaturated, or polyunsaturated
Analyzing Lipid Metabolism: Activation and -Oxidation of Fatty Acids
There is massive gene replication predicted for the activation of fatty acids and their entry into the β-oxidation cycle for fatty acid oxidation. These two steps in fatty acid metabolism are catalyzed by FadD and FadE enzymes with 36 genes
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