- ￥2890
- LSM Bio
- 参照说明书
- 0.31-20ng/ml
- 国产
- ELI-11690h
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
参照说明书
- 检测方法：
夹心法/竞争法
- 应用：
0.1ng/ml
- 适应物种：
人
- 标记物：
Protein arginine N- methyltransferase 8, PRMT8
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.31-20ng/ml
- 规格：
96T
Human Protein arginine N- methyltransferase 8, PRMT8 ELISA KIT
Product Name:Human Protein arginine N- methyltransferase 8, PRMT8 ELISA KIT
Packing:96T
Catalog No.:ELI-11690h
Gene Name:PRMT8
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Protein arginine N-methyltransferase 8(PRMT8
Alternative Name:PRMT8,Protein arginine N-methyltransferase 8(PRMT8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Protein arginine N- methyltransferase 8, PRMT8 ELISA KIT allows for the in vitro quantitative determination of Protein arginine N-methyltransferase 8(PRMT8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Protein arginine N- methyltransferase 8, PRMT8 ELISA KIT has been pre-coated with an Protein arginine N-methyltransferase 8(PRMT8 antibody specific to Protein arginine N-methyltransferase 8(PRMT8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Protein arginine N-methyltransferase 8(PRMT8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Protein arginine N-methyltransferase 8(PRMT8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Protein arginine N-methyltransferase 8(PRMT8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation
Functional Assays on High‐Content Protein Microarrays
Substrate Profiling Assay on the ProtoArray Human Protein Array Basic Protocol 5: Methyltransferase Profiling Assay on the ProtoArray Human Protein Array Reagents and Solutions Commentary
An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between n-6 fatty acid (FA)-derived lipid peroxide and lysine. Immunochemical and chemical methods can be used to detect the formation of HEL. For example, an ELISA
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