- 询价
- Signalway Antibody
- C48107
- 2025年09月23日
- WB, IF
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
peptide
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB, IF
- 抗体英文名：
DYKDDDDK Tag(FLAG) Conjugated Antibody
- 规格：
50ul/100ul
DDDK antibody
ddk antibody
DYKDDDDK antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
FLAG antibody
FLAG tag antibody
配方:0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide
应用详情:WB: 1:50-1:200
IF:1:50-1:200
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
1. Purifying DYKDDDDK-tag fusion proteins from crude E.coli extracts. 1) Grow the bacteria cells under the condition that induce production of DYKDDDDK-tag fusion proteins. 2) Harvest the cells by centrifugation at 10,000 rpm for 20
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
Figure 19.20.1 The Strep/FLAG tandem affinity purification. (A ) N‐ and C‐terminal SF‐TAP tags (POI, protein of interest). (B ) Overview of both purification steps. (1) Purification by the tandem Strep‐tag II moiety: binding to Strep‐Tactin matrix
0.1% Tween-20 and 0.2% I-Block), incubate 30 min, room temperature, gently rocking. Wash three times in 20 ml 1X Western buffer (containing 0.1% Tween-20 and 0.2% I-Block) for 5 min each. Add appropriate dilution of secondary antibody conjugated
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