- 询价
- Signalway Antibody
- 48107
- 2025年09月23日
- WB, IP
- Mouse
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 免疫原：
peptide
- 形态：
liquid
- 保存条件：
Store at -20˚C
- 克隆性：
Monoclonal
- 保质期：
12 months
- 抗原来源：
Mouse
- 供应商：
南京赛戈巍生物科技有限公司
- 宿主：
Mouse
- 应用范围：
WB, IP
- 抗体英文名：
DYKDDDDK Tag(FLAG) Antibody
- 规格：
50ul/100ul
DDDK antibody
ddk antibody
DYKDDDDK antibody
DYKDDDDK epitope tag antibody
DYKDDDDK tag antibody
ECS epitope tag antibody
ECS tag antibody
Enterokinase Cleavage Site epitope tag antibody
Enterokinase Cleavage Site tag antibody
FLAG antibody
FLAG tag antibody
配方:1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
应用详情:WB: 1:5,000-1:10,000
IP: 1:10-1:50
图片:
Western blot analysis on DYKDDDDK Tag recombinant protein (65kDa).
,
Immunoprecipitation analysis of different lysates using anti-FLAG antibody. Lane 1: Non-transfected 293T Lane 2: Transfected 293T Lane 3: Agarose beads complexes Lane 4: Negative control (Normal mouse IgG)
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- 作者
- 内容
- 询问日期
文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
1. Purifying DYKDDDDK-tag fusion proteins from crude E.coli extracts. 1) Grow the bacteria cells under the condition that induce production of DYKDDDDK-tag fusion proteins. 2) Harvest the cells by centrifugation at 10,000 rpm for 20
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
Purification and Characterization of His-Tagged Antibody Fragments
, typically His6 , fused to the recombinant protein. In the case of antibody fragments that are composed of different chains, e.g., Fv and Fab, the site of attachment for the His6 tag has to be carefully chosen, and mild chromatographic conditions
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