BstFNI内切酶北京品牌

特别提示：包括BstFNI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BstFNI内切酶
英文名称：BstFNI
产品货号：GS2196
产品规格：300U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl
Recognition sites	CG↑CG
来源	Bacillus stearothermophilus FN
Assayed on	Lambda DNA
Optimal buffer	Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.)
Optimal temperature	60℃
Storage conditions	20 mM Tris-HCl(pH 7.5);300 mM NaCl;0.1 mM EDTA;10 mM MgCl2;200 μg/ml BSA,7 mM 2-mercaptoethanol and 50%glycerol;Store at -20℃.
Ligations	After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 60℃.
Methlation effect	Blocked by CG methylation
Thermal inactivation	enzyme is inactivated by incubation at 80℃ for 20 mins.
Campatible Ends	AccII,Bsh1236I,BspFNI^,BstUI,FnuDII^,MvnI,ThaI

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