PspCI内切酶现货供应

特别提示：包括PspCI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：PspCI内切酶
英文名称：PspCI
产品货号：GS2221
产品规格：500U

储存条件：冷冻（-20℃）

使用说明

To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	CAC↑GTG
来源	Pseudomonas species C
Assayed on	Lambda DNA
Optimal buffer	B?(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;1 mM DTT.)+?BSA
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;10mM2-mercaptoethanol;200 μg/ml BSA;and 50%glycerol.|Storage temperature:-20℃.|For long term storage(more than 30 days),store at -70oC.
Ligations	After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.Ligation is better in presence of 10%PEG.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	AcvI,BbrPI,Eco72I,PmaCI,PmlI

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