BamHI内切酶北京现货

特别提示：包括BamHI内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：BamHI内切酶
英文名称：BamHI
产品货号：GS2072
产品规格：2kU

储存条件：冷冻（-20℃）

使用说明

1.High enzyme concentration may result in star activity.2.Do not use BSA for long incubation.3.To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	G↑GATCC
来源	E.coli strain,that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Assayed on	Lambda DNA
Optimal buffer	10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;50 mM NaCl;1 mM DTT.+BSA.
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.6);50 mM NaCl;0.1 mM EDTA;100μg/ml BSA;1 mM DTT;50%glycerol.
Ligations	After 50-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Not blocked by Dam methylation(GmATC)GGATCC.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
兼容的末端	BclI,BglII,Bsp143I,MboI,PsuI.

我公司销售的BamHI内切酶北京现货，BamHI限制性内切酶质量上佳，价格普惠，欢迎广大新老客户踊跃订购。